Cells were extracted using the lamina propria dissociation kit (Miltenyi Biotech). Cells were crosslinked and FACS sorted into MACS buffer and Chromatin was analysed using iChIP (Lara-Aatiaso et al.) with our new modification iChIP-IVT protocol developed by (Lara-Astiaso et al.) with our modification iChIP-IVT.