GSM2257573: HELA cell RNA PolII CHIP-seq with ERK inhibitor treatment; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
cervix
cell line
HeLa
genetic background
Harboring inducible shRNA expressing cassette
culture medium
Starvation: Culture in 0.5% FBS medium for 2 days
agent
ERK inhibitor 3 hours at 1uM
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin associate RNA were extracted from cultured cells for RNA-seq. DNA was immuno-precipitated with antibody from sonicated cell lysate. RNA and CHIP libraries were prepared with NEBNext Library Prep Kits for Illumina using standard Illumina protocols