GSM7165519: IP NT2 16hour retinoic acid rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NUTM1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
NTERA-2
Primary Tissue
Testis
Site of Extraction
lung
Tissue Diagnosis
Carcinoma, Embryonal
Attributes by original data submitter
Sample
source_name
Cell state-dependent chromatin targeting in NUT carcinoma
cell line
Ntera-2 derived differentiated cells
cell type
human embryonal carcinoma cell line
chip antibody
NUT (C52B1) Rabbit mAb#3625
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7165519
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked chromatin for ChIP-seq experiments with antibodies against were prepared from 5 × 107 transient transfected cells of each HUES64, HUEs64 derived mesoderm, Ntera-2 and Ntera-2 derived differentiated cells as described in Alekseyenko et al. 2015. ChIP-seq was performed using anti-NUT (15 ul per IP; Cell Signaling, catalog no. 3625).ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015). ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015).