Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Neural

Cell type

Cerebellum

MeSH Description

The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Sample

source_name

whole cerebellum

strain

C57BL/6

developmental stage

Postnatal day 60 (P60)

antibody

H3K27Ac, Abcam Ab4729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin Immunoprecipitation (anti histone H3K27Ac, Abcam Ab4729). NEB Library Preparation Kit ( NEB 6240S and NEB E7335s)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

27242397

Reads aligned (%)

98.1

Duplicates removed (%)

12.1

Number of peaks

34952 (qval < 1E-05)

mm9

Number of total reads

27242397

Reads aligned (%)

98.1

Duplicates removed (%)

12.2

Number of peaks

34936 (qval < 1E-05)