GSM2255826: 28 BeWo ZSCAN5D; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ZSCAN5D
Cell type
Cell type Class
Placenta
Cell type
BeWo
Primary Tissue
Placenta
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Human BeWo cell line
cell line
Human BeWo
antibody
ZSCAND
treatment or control
treatment
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was prepared and sonicated using Bioruptor UCD-200 in ice water bath to generate DNA fragments 200-300 bp in size. Twenty micrograms of each antibody preparation, or 20 µg IgG for mock pulldown controls, were incubated with chromatin prepared from nuclei of approximately 5 million cells. ChIP-seq libraries were generated using KAPA LTP Library Preparation Kits (Kapa Biosystems, KK8232) to yield two independent ChIP replicates for the antibody. We also generated libraries from sonicated genomic input DNA from the same chromatin preparations as controls. Libraries were bar-coded with Bioo Scientific index adapters and sequenced to generate 15-23 million reads per duplicate sample using the Illumina Hi-Seq 2000 instrument at the University of Illinois W.M. Keck Center for Comparative and Functional Genomics according to manufacturer’s instructions.