Antigen

Antigen Class

TFs and others

Antigen

Pcgf6

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell line

CCE

passages

<15

chip antibodies

Pcgf6 (Santa Cruz, sc-160649)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear extracts were prepared using cell extraction buffers and sonication. Specific histone-DNA complexes were isolated using Pcgf6 antibodies. Pcgf6-bound genomic DNA was extracted and amplified using an Illumina Library Prep Kit (Illumina, IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

62902088

Reads aligned (%)

96.8

Duplicates removed (%)

88.6

Number of peaks

490 (qval < 1E-05)

mm9

Number of total reads

62902088

Reads aligned (%)

96.6

Duplicates removed (%)

88.7

Number of peaks

493 (qval < 1E-05)