Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Cdx2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
EpiSC
NA
NA

Attributes by original data submitter

Sample

source_name
Cdx2 wtEpiSCs 24h rep2
tissue
EpiSCs
antibody
Biogenex, MU392A-UC (CDX2-88)
treatment
24h CHIR99021 + Fgf8

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinking 15mins RT, lysis, sonication and immunoprecipiation following standard procedures. Illumina Truseq DNA library protocol

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
102557467
Reads aligned (%)
93.5
Duplicates removed (%)
35.3
Number of peaks
1249 (qval < 1E-05)

mm9

Number of total reads
102557467
Reads aligned (%)
93.2
Duplicates removed (%)
35.3
Number of peaks
1263 (qval < 1E-05)

Base call quality data from DBCLS SRA