Antigen

Antigen Class

Histone

Antigen

H3K36me2

Cell type

Cell type Class

Prostate

Cell type

PC-3

Primary Tissue

Prostate

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

shWHSC1 H3K36me2 ChIPSeq

cell line

PC3

tissue

prostate; derived from metastatic site: bone

chip antibody

H3K36me2(Millipore)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against H3K36me2( Millipore). Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

35176242

Reads aligned (%)

97.7

Duplicates removed (%)

3.4

Number of peaks

1832 (qval < 1E-05)

hg19

Number of total reads

35176242

Reads aligned (%)

97.3

Duplicates removed (%)

4.4

Number of peaks

1818 (qval < 1E-05)