Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me2

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
control H3K36me2_ChIPSeq
cell line
PC3
tissue
prostate; derived from metastatic site: bone
chip antibody
H3K36me2(Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against H3K36me2( Millipore). Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
34337607
Reads aligned (%)
97.7
Duplicates removed (%)
3.4
Number of peaks
1500 (qval < 1E-05)

hg19

Number of total reads
34337607
Reads aligned (%)
97.1
Duplicates removed (%)
5.1
Number of peaks
1379 (qval < 1E-05)

Base call quality data from DBCLS SRA