The hollow, muscular organ that maintains the circulation of the blood.
Attributes by original data submitter
Sample
source_name
HF_STZ_neonatal heart
strain
Sprague-Dawley
maternal diet/treatment
high fat diet (HF) + Streptozotocin (STZ, 65 mg/Kg)
developmental stage
neonatal (pups delivered on gestational day 21)
tissue
heart
chip antibody
H3K4me3 (Epigentek, A-4033)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Five to six frozen heart tissues were pooled to make >250mg/sample and homogenized followed by chromatin isolation using P-2001 ChromaFlashTM Chromatin Extraction Kit (Epigentek, Farmingdale, NY). Chromatin was sheared using Episonic2000 Sonication System (Epigentek, Farmingdale, NY) in 300 μl of ChIP buffer. DNA fragment (100-300 bp) was quality-checked using Bioanalyzer analysis. A ChIP-sequencing library was prepared using the EpiNext ChIP-Seq High Sensitivity Kit with amplification, DNA end polishing and adaptor ligation. 10 nM of sample libraries were provided for next generation sequencing on a HiSeq 2500.