Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

Immortalized human astrocytes (Sanoda 2001)

chip antibody

EZH2 (Cat. 39002, Active Motif)

treatment

treated with CRISPRa system with Control sgRNAs for 14 days

cell type

Immortalized human astrocytes (Sanoda 2001)

Sequenced DNA Library

library_name

GSM7108393

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB) Libraries were prepared according to Illumina's instructions accompanying NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S, NEB).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

27668723

Reads aligned (%)

98.9

Duplicates removed (%)

0.3

Number of peaks

1512 (qval < 1E-05)

hg19

Number of total reads

27668723

Reads aligned (%)

98.3

Duplicates removed (%)

0.2

Number of peaks

1581 (qval < 1E-05)