Cells were lysed by vortexing with glass beads in the presence of protease inhibitors. The samples were then sonicated four times for 10 seconds to shear the DNA. Samples were pre-cleared using protein A agarose beads for 30 minutes with rotation at 4C. Samples were then incubated at 4C overnight with 5µL of anti-HA (Sigma H6908). Sir2-HA and Hst1-HA were pulled down using protein A agarose beads. The DNA was eluted from the beads and crosslinking was reversed at 65C overnight. The DNA was extracted with phenol chloroform. The DNA was resuspended in 25 uM tris. ChIP samples were sent for ChIP-seq library preparation and 50 cycle single read sequencing on an Illumina HiSeq2500 at The University at Buffalo Genomics and Sequencing Core.