GSM7093735: T-47D c-JUN DMSO; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
JUN
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T-47D
cell line
T-47D
cell type
Breast; Ductal Carcinoma
chip antibody
c-JUN (BD Biosciences 558036)
treatment
DMSO (veh)
time
1 hour
Sequenced DNA Library
library_name
GSM7093735
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction was performed as described in Reddy et al, Genome Research, 2009.