Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYF5

Cell type

Cell type Class
Muscle
Cell type
RH-18
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
RH18 Fusion Negative Rhabdomyosarcoma cell line
antibody
Myf-5, Santa Cruz (C-20) cat# sc-302
enrichment target
MYF5
target function
Transcription Factor

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
35031306
Reads aligned (%)
74.7
Duplicates removed (%)
22.3
Number of peaks
16802 (qval < 1E-05)

hg38

Number of total reads
35031306
Reads aligned (%)
76.2
Duplicates removed (%)
21.2
Number of peaks
17038 (qval < 1E-05)

Base call quality data from DBCLS SRA