GSM2242173: siControl anti-VRK1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
VRK1
Cell type
Cell type Class
Bone
Cell type
HT-1080
Primary Tissue
Bone
Tissue Diagnosis
Fibrosarcoma
Attributes by original data submitter
Sample
source_name
fibrosarcoma
cell line
HT1080
chip antibody
anti-VRK1
sirna
siControl
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates derived from fixed and sonicated nuclei was immunoprecipitated by anti-H2ApT120, anti-H2AubK119 and anti-VRK1 antibody. To add single adenosine, DNA was treated with klewnow exo- (3’ and 5’ exo minus; Illumina). Illumina adaptors were added and subjected to 20 cycles of PCR amplification according to manufacturer's instructions. Purified DNA was subjected to cluster generation and 36 cycles of sequencing on the Illumina cluster station and Genome Analyzer following the manufacturer’s instractions.