GSM2242173: siControl anti-VRK1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
Lysates derived from fixed and sonicated nuclei was immunoprecipitated by anti-H2ApT120, anti-H2AubK119 and anti-VRK1 antibody. To add single adenosine, DNA was treated with klewnow exo- (3’ and 5’ exo minus; Illumina). Illumina adaptors were added and subjected to 20 cycles of PCR amplification according to manufacturer's instructions. Purified DNA was subjected to cluster generation and 36 cycles of sequencing on the Illumina cluster station and Genome Analyzer following the manufacturer’s instractions.