GSM7081489: HepG2 cells, IP928-1XBP1s (ChIP-seq); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
XBP1
Cell type
Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular
Attributes by original data submitter
Sample
source_name
HepG2
cell line
HepG2
cell type
human HCC cell line
treatment
Thapsigargin
chip antibody
anti-XBP1s (BioLegend, #647502)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and XBP-1-DNA complexes were isolated with anti-XBP1s (BioLegend， #647502). Reads were filtered using TrimGalore software and duplicate reads were removed. Sequencing data were aligned to the hg38 reference genome. The NEXTFLEX® ChIP-Seq Library Prep Kit was used for library generation according to Illumina's protocol.