Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD4

Cell type

Cell type Class
Neural
Cell type
SK-N-BE(2)
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
bone marrow metastatic neuroblastoma
cell type
neuroblastoma cell line
cell line
SK-N-BE2
chip antibody
anti-TEAD4 (ab58310; abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed for protein, with subsequent protein quantified by spectrophotometry. Protein was resolved on 8–14% Tris-Bis gels, transferred to PVDF membranes, blocked and subjected to primary and secondary antibodies

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
79871763
Reads aligned (%)
58.3
Duplicates removed (%)
30.0
Number of peaks
44022 (qval < 1E-05)

hg38

Number of total reads
79871763
Reads aligned (%)
58.9
Duplicates removed (%)
29.6
Number of peaks
43984 (qval < 1E-05)

Base call quality data from DBCLS SRA