Antigen

Antigen Class

TFs and others

Antigen

TEAD4

Cell type

Cell type Class

Neural

Cell type

SK-N-BE(2)

Primary Tissue

Brain

Site of Extraction

Bone Marrow

Tissue Diagnosis

Neuroblastoma

Sample

source_name

bone marrow metastatic neuroblastoma

cell type

neuroblastoma cell line

cell line

SK-N-BE2

chip antibody

anti-TEAD4 (ab58310; abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were lysed for protein, with subsequent protein quantified by spectrophotometry. Protein was resolved on 8–14% Tris-Bis gels, transferred to PVDF membranes, blocked and subjected to primary and secondary antibodies

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

79871763

Reads aligned (%)

83.7

Duplicates removed (%)

29.6

Number of peaks

43984 (qval < 1E-05)

hg19

Number of total reads

79871763

Reads aligned (%)

83.3

Duplicates removed (%)

30.0

Number of peaks

44022 (qval < 1E-05)