Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
B6 X BCA F1 ESC line - Addgene 52382, H3K27me3 and H3K4me3 ChIPs
strain/background
B6 X BCA F1
tissue
Embryonic stem cell line
sample type
cell line
chip antibody1
H3K27me3 (Millipore 07-449)
chip antibody2
H3K4me3 (Millipore 07-473)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
2593779
Reads aligned (%)
84.1
Duplicates removed (%)
5.4
Number of peaks
116 (qval < 1E-05)

mm9

Number of total reads
2593779
Reads aligned (%)
83.9
Duplicates removed (%)
5.5
Number of peaks
120 (qval < 1E-05)

Base call quality data from DBCLS SRA