Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

B6 X BCA F1 ESC line - Addgene 52382, H3K27ac and H3K4me3 ChIPs

strain/background

B6 X BCA F1

tissue

Embryonic stem cell line

sample type

cell line

chip antibody1

H3K27ac (Abcam ab4729)

chip antibody2

H3K4me3 (Millipore 07-473)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

7009801

Reads aligned (%)

83.5

Duplicates removed (%)

6.5

Number of peaks

13247 (qval < 1E-05)

mm9

Number of total reads

7009801

Reads aligned (%)

83.3

Duplicates removed (%)

6.6

Number of peaks

13215 (qval < 1E-05)