Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

Bone marrow-derived dendritic cells, H4K5ac and H3K4me2 ChIPs

strain/background

C57BL/6

tissue

Bone marrow-derived dendritic cells

sample type

primary culture

chip antibody1

H4K5ac (Millipore 07-327)

chip antibody2

H3K4me2 (Abcam ab32356)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

4284648

Reads aligned (%)

97.9

Duplicates removed (%)

32.2

Number of peaks

3083 (qval < 1E-05)

mm9

Number of total reads

4284648

Reads aligned (%)

97.8

Duplicates removed (%)

32.2

Number of peaks

3147 (qval < 1E-05)