Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver, H3K27me3 and H3K4me1 ChIPs

strain/background

C57BL/6

tissue

Liver

age

Adult

chip antibody1

H3K27me3 (Millipore 07-449)

chip antibody2

H3K4me1 (Abcam ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

26211258

Reads aligned (%)

96.7

Duplicates removed (%)

47.9

Number of peaks

2451 (qval < 1E-05)

mm9

Number of total reads

26211258

Reads aligned (%)

96.5

Duplicates removed (%)

48.0

Number of peaks

2392 (qval < 1E-05)