GSM2231417: ChIP-seq BRG1 T30; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMARCA4
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
Breast cancer cells
cell line
T47D
small interfering knock-down
none
time after r5020
30 minutes
chip antibody
anti_BRG1 Abcam ab110641 [EPNCIR111A]
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq and Mnase-seq experiments were performed according to Vicent et al., Methods Mol Biol. 2014;1204:1-14. Book “Steroid receptors: Methods and protocols”, Humana press (Springer, U.S.A.) Progesterone receptor interaction with chromatin We have used the NEBNext Ultra DNA Library Prep kit for Illumina (Ref.:#7370), with amplification by Real Time PCR (light cycler) to determine the optimal number of cycles to amplify the library