Sample information curated by ChIP-Atlas

Antigen

Antigen Class
DNase-seq
Antigen
DNase-Seq

Cell type

Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)

Attributes by original data submitter

Sample

source_name
mG/ER
biomaterial_provider
Stam Lab
lab
UW-m
lab description
Stamatoyannopoulous - University of Washington
datatype
DnaseSeq
datatype description
DNaseI HS Sequencing
cell
mG/ER
cell organism
mouse
cell description
MEL-GATA-1-ER, This is a mouse suspension cell line derived from MEL cells by stable transfection with a GATA-1-ER fusion protein construct as described by Choe et al., 2003 (Cancer Res 63, 6363-6369, 2003). These cells can be terminally differentiated into mature erythroid cells with ?-estradiol treatment
cell sex
M
age
adult-8wks
age description
Adult 8 weeks
treatment
diffProtC_24hr
treatment description
24 hour differentiation time point of mG/ER cells towards red blood cells after treatment with estradiol. Estradiol 10^-7 M final concentration (Stam)
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse
age
adult-8wks
age description
Adult 8 weeks
labexpid
DS15410,DS15405
labversion
1,Hotspot-v5.2,lmax-v1.1,WindowDensity-bin20-win+/-75
replicate
1,2
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1014181: UW_DnaseSeq_mG/ER_adult-8wks_diffProtC_24hr_C57BL/6
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
84612445
Reads aligned (%)
95.5
Duplicates removed (%)
49.2
Number of peaks
62173 (qval < 1E-05)

mm9

Number of total reads
84612445
Reads aligned (%)
95.4
Duplicates removed (%)
49.2
Number of peaks
62228 (qval < 1E-05)

Base call quality data from DBCLS SRA