Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

GEO Accession

GSM1010891

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

antibody vendorname

David Anderson, Caltech

antibody

NRSF

cell description

mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

ectoderm

cell organism

human

cell sex

F

cell

MCF-7

controlid

SL13504

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL14698,SL13491

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010891: HudsonAlpha_ChipSeq_MCF-7_NRSF_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

59354697

Reads aligned (%)

82.4

Duplicates removed (%)

12.2

Number of peaks

14691 (qval < 1E-05)

hg38

Number of total reads

59354697

Reads aligned (%)

83.7

Duplicates removed (%)

11.2

Number of peaks

14347 (qval < 1E-05)