Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

GEO Accession
GSM1010843
antibody antibodydescription
Rabbit Polyclonal IgG. Antibody Target: PU1
antibody targetdescription
PU.1 (H-135)
antibody vendorid
sc-22805
antibody vendorname
Santa Cruz Biotechnology
antibody
PU.1
cell description
promyelocytic leukemia cells, (PMID: 276884)
cell karyotype
cancer
cell lineage
mesoderm
cell organism
human
cell sex
F
cell
HL-60
controlid
SL12792,SL16759
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL12629,SL14712
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010843: HudsonAlpha_ChipSeq_HL-60_PU.1_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
49466528
Reads aligned (%)
79.7
Duplicates removed (%)
14.0
Number of peaks
88492 (qval < 1E-05)

hg38

Number of total reads
49466528
Reads aligned (%)
81.1
Duplicates removed (%)
13.0
Number of peaks
88800 (qval < 1E-05)

Base call quality data from DBCLS SRA