Antigen

Antigen Class

TFs and others

Antigen

TAF1

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

GEO Accession

GSM1010812

antibody antibodydescription

Mouse monoclonal (p250). Antibody Target: TAF1

antibody targetdescription

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

antibody vendorid

sc-735

antibody vendorname

Santa Cruz Biotechnology

antibody

TAF1

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

endoderm

cell organism

human

cell sex

M

cell

A549

controlid

SL6021

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL5358,SL5700

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

1 h with 0.02% Ethanol (Myers)

treatment

EtOH_0.02pct

Sequenced DNA Library

library_name

GSM1010812: HudsonAlpha_ChipSeq_A549_TAF1_EtOH_0.02pct_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

86273191

Reads aligned (%)

64.5

Duplicates removed (%)

11.6

Number of peaks

11196 (qval < 1E-05)

hg38

Number of total reads

86273191

Reads aligned (%)

65.9

Duplicates removed (%)

10.4

Number of peaks

11296 (qval < 1E-05)