GSM6932410: MCF7 ER ChIP rep 1 - dex; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF-7
cell type
Breast Cancer Cells
cell line
MCF-7
genotype
WT
treatment
Vehicle
time point
2h
chip antibody
ER (06-935, Merck)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6932410
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation followed by sequencing (ChIP)-seq was performed as previously described (Schmidt, D. et al. Methods 48, 240–248 (2009).In brief, cells were fixed in 1% formaldehyde for 10 min and quenched with 0.125M glycine. Nuclear lysates were extracted as previously described and sonicated for 13 cycles (30 sec on/30 sec off) using the Bioruptor® Pico (B01060001, Diagenode). ). Per ChIP, 50 µL of magnetic Protein A beads (10008D, Thermo Fisher Sci-entific) beads were conjugated to 7.5µL of GR antibody, or 5µg of ER antibody. Immunoprecipitated DNA was processed for library preparation usin KAPA library preparation kit, KK8234, Roche.