Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL3

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010775
antibody antibodydescription
Bcl-3(C-14) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: BCL3
antibody targetdescription
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. (Provided by RefSeq).
antibody vendorid
sc-185
antibody vendorname
Santa Cruz Biotechnology
antibody
BCL3
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
A549
controlid
SL6021
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL6596,SL7109
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
1 h with 0.02% Ethanol (Myers)
treatment
EtOH_0.02pct

Sequenced DNA Library

library_name
GSM1010775: HudsonAlpha_ChipSeq_A549_BCL3_EtOH_0.02pct_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
93341655
Reads aligned (%)
67.4
Duplicates removed (%)
9.3
Number of peaks
13325 (qval < 1E-05)

hg38

Number of total reads
93341655
Reads aligned (%)
68.5
Duplicates removed (%)
8.5
Number of peaks
13400 (qval < 1E-05)

Base call quality data from DBCLS SRA