Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

GEO Accession
GSM1010744
antibody antibodydescription
Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
antibody vendorname
David Anderson, Caltech
antibody
NRSF
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
cell organism
human
cell sex
F
cell
GM12878
controlid
SL1394,SL3979
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL7333,SL852
protocol description
one 15-cycle round of PCR (Myers)
protocol
PCR1x
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010744: HudsonAlpha_ChipSeq_GM12878_NRSF_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
45419808
Reads aligned (%)
63.7
Duplicates removed (%)
13.9
Number of peaks
6801 (qval < 1E-05)

hg38

Number of total reads
45419808
Reads aligned (%)
65.2
Duplicates removed (%)
14.4
Number of peaks
6478 (qval < 1E-05)

Base call quality data from DBCLS SRA