Antigen

Antigen Class

TFs and others

Antigen

ATF2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

GEO Accession

GSM1010742

antibody antibodydescription

Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of ATF2 of human origin. Antibody Target: ATF2

antibody targetdescription

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. (provided by RefSeq)

antibody vendorid

sc-81188

antibody vendorname

Santa Cruz Biotechnology

antibody

ATF2_(SC-81188)

cell description

embryonic stem cells

cell karyotype

normal

cell lineage

inner cell mass

cell organism

human

cell sex

M

cell

H1-hESC

controlid

SL6018

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL7904,SL8136

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010742: HudsonAlpha_ChipSeq_H1-hESC_ATF2_(SC-81188)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

81021638

Reads aligned (%)

86.6

Duplicates removed (%)

7.9

Number of peaks

19692 (qval < 1E-05)

hg19

Number of total reads

81021638

Reads aligned (%)

86.3

Duplicates removed (%)

8.3

Number of peaks

19493 (qval < 1E-05)