Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

K562

cell organism

human

cell description

leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

cell karyotype

cancer

cell lineage

mesoderm

cell sex

F

antibody

H3K36me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K36me3

antibody targetdescription

Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.

antibody vendorname

Abcam

antibody vendorid

ab9050

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000471

labexpid

DS12067

labversion

Bowtie 0.12.5

replicate

1

Sequenced DNA Library

library_name

GSM945302: UW_ChipSeq_K562_H3K36me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

36670258

Reads aligned (%)

96.9

Duplicates removed (%)

7.2

Number of peaks

562 (qval < 1E-05)

hg19

Number of total reads

36670258

Reads aligned (%)

96.3

Duplicates removed (%)

8.3

Number of peaks

615 (qval < 1E-05)