Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Lung
Cell type
HPF
Tissue
lung
Description
pulmonary fibroblasts isolated from lung tissue

Attributes by original data submitter

Sample

source_name
HPF
biomaterial_provider
ScienCell
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HPF
cell organism
human
cell description
pulmonary fibroblasts isolated from lung tissue
cell karyotype
normal
cell sex
U
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000960
labexpid
DS16184
labversion
Bowtie 0.12.7
replicate
1

Sequenced DNA Library

library_name
GSM945284: UW_ChipSeq_HPF_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
49955724
Reads aligned (%)
97.2
Duplicates removed (%)
26.9
Number of peaks
41069 (qval < 1E-05)

hg19

Number of total reads
49955724
Reads aligned (%)
96.9
Duplicates removed (%)
27.4
Number of peaks
41019 (qval < 1E-05)

Base call quality data from DBCLS SRA