Antigen

Antigen Class

TFs and others

Antigen

IRF9

Cell type

Cell type Class

Liver

Cell type

Huh-7.5

Tissue

liver

Lineage

endoderm

Description

hepatocellular carcinoma, hepatocytes selected for high levels of hepatitis C replication

Sample

source_name

Huh7.5

cell line

Huh7.5

cell type

human hepatocellular carcinoma cells

chip antibody

IRF9 (CST, #76684)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM6928597

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was carried out as previously described (Piaszyk-Borychowska et al. 2012) ChIP-seq libraries were prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® (E6240, NEB) and NEBNext® Multiplex Oligos for Illumina®, according to the manufacturer's protocol. Libraries were validated using the Agilent High Sensitivity DNA Kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

14071281

Reads aligned (%)

98.3

Duplicates removed (%)

14.1

Number of peaks

467 (qval < 1E-05)

hg19

Number of total reads

14071281

Reads aligned (%)

97.8

Duplicates removed (%)

14.9

Number of peaks

506 (qval < 1E-05)