Antigen

Antigen Class

TFs and others

Antigen

STAT2

Cell type

Cell type Class

Liver

Cell type

Huh-7.5

Tissue

liver

Lineage

endoderm

Description

hepatocellular carcinoma, hepatocytes selected for high levels of hepatitis C replication

Sample

source_name

Huh7.5

cell line

Huh7.5

cell type

human hepatocellular carcinoma cells

chip antibody

phospho-STAT2 (CST, #88410)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM6928590

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was carried out as previously described (Piaszyk-Borychowska et al. 2012) ChIP-seq libraries were prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® (E6240, NEB) and NEBNext® Multiplex Oligos for Illumina®, according to the manufacturer's protocol. Libraries were validated using the Agilent High Sensitivity DNA Kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

14916978

Reads aligned (%)

92.5

Duplicates removed (%)

19.5

Number of peaks

23899 (qval < 1E-05)

hg19

Number of total reads

14916978

Reads aligned (%)

92.0

Duplicates removed (%)

20.2

Number of peaks

23830 (qval < 1E-05)