Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Neural

Cell type

SK-N-SH

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SK-N-SH_RA

biomaterial_provider

ATCC

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

SK-N-SH_RA

cell organism

human

cell description

neuroblastoma cell line, treatment: differentiated with retinoic acid, (Biedler, et al. Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture. Cancer Research 33, 2643-2652, November 1973.)

cell karyotype

cancer

cell lineage

ectoderm

cell sex

F

antibody

H3K27me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27me3

antibody targetdescription

Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

antibody vendorname

Millipore

antibody vendorid

07-449

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000474

labexpid

DS12212

labversion

Bowtie 0.12.5

replicate

1

Sequenced DNA Library

library_name

GSM945210: UW_ChipSeq_SK-N-SH_RA_H3K27me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

33520809

Reads aligned (%)

95.4

Duplicates removed (%)

4.3

Number of peaks

874 (qval < 1E-05)

hg19

Number of total reads

33520809

Reads aligned (%)

94.8

Duplicates removed (%)

5.3

Number of peaks

923 (qval < 1E-05)