Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

GM06990

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus transformed

Sample

source_name

GM06990

biomaterial_provider

GM06990

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

GM06990

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus transformed

cell karyotype

normal

cell lineage

mesoderm

cell sex

F

antibody

H3K27me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27me3

antibody targetdescription

Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

antibody vendorname

Millipore

antibody vendorid

07-449

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000455

labexpid

DS12191

labversion

Bowtie 0.12.5

replicate

1

Sequenced DNA Library

library_name

GSM945205: UW_ChipSeq_GM06990_H3K27me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

32205959

Reads aligned (%)

95.4

Duplicates removed (%)

3.3

Number of peaks

148 (qval < 1E-05)

hg19

Number of total reads

32205959

Reads aligned (%)

94.4

Duplicates removed (%)

3.7

Number of peaks

560 (qval < 1E-05)