Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Epidermis
Cell type
NHEK
Tissue
skin
Lineage
ectoderm
Description
epidermal keratinocytes

Attributes by original data submitter

Sample

source_name
NHEK
biomaterial_provider
Lonza
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
NHEK
cell organism
human
cell description
epidermal keratinocytes
cell karyotype
normal
cell lineage
ectoderm
cell sex
U
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000472
labexpid
DS12237
labversion
Bowtie 0.12.5
replicate
1

Sequenced DNA Library

library_name
GSM945174: UW_ChipSeq_NHEK_H3K36me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
46699601
Reads aligned (%)
93.0
Duplicates removed (%)
9.3
Number of peaks
781 (qval < 1E-05)

hg19

Number of total reads
46699601
Reads aligned (%)
92.6
Duplicates removed (%)
9.9
Number of peaks
789 (qval < 1E-05)

Base call quality data from DBCLS SRA