Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Lung
Cell type
AG04450
Tissue
lung
Lineage
endoderm
Description
fetal lung fibroblast

Attributes by original data submitter

Sample

source_name
AG04450
biomaterial_provider
Coriell
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
AG04450
cell organism
human
cell description
fetal lung fibroblast
cell karyotype
normal
cell lineage
endoderm
cell sex
M
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
AG04450/None/Input/std
labexpid
DS21480
labversion
Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75
replicate
1

Sequenced DNA Library

library_name
GSM1010913: UW_ChipSeq_AG04450_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
20752311
Reads aligned (%)
75.4
Duplicates removed (%)
1.7
Number of peaks
136 (qval < 1E-05)

hg19

Number of total reads
20752311
Reads aligned (%)
75.0
Duplicates removed (%)
2.3
Number of peaks
173 (qval < 1E-05)

Base call quality data from DBCLS SRA