GSM6922376: RPE TP53-/-, control, CTCF, mouse spike-in; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Neural
Cell type
hTERT RPE-1
Primary Tissue
Eye
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
retinal epithelium
tissue
retinal epithelium
cell line
hTERT RPE-1
cell type
immortalized retinal epithelial cells
genotype
TP53-/-
chip antibody
CTCF
condition
control
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, catalogue no. 91820S) following the manufacturer's protocol. Chromatin immunoprecipitation was obtained by incubating chromatin at 4°C overnight with either of the following antibodies, at the recommended dilutions: anti-Acetyl-Histone H3 (Lys27) (Cell Signaling Technology, catalogue no. 8173), anti-Tri-Methyl-Histone H3 (Lys9) (Cell Signaling Technology, catalogue no. 13969), anti-CTCF (Active Motif, catalogue no. 61311), anti-Tri-Methyl-Histone H3 (Lys4) (Cell Signaling Technology, catalogue no. 9751), Tri-Methyl-Histone H3 (Lys27) (Cell Signaling Technology, catalogue no. 9733). Library preparation for sequencing from the chromatin immunoprecipitated DNA was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs, catalogue no. E7645) following manufacturer's protocol.