Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Epidermis

Cell type

Fibrobl GM03348

Tissue

skin

Description

skin fibroblast

Sample

source_name

Fibrobl_GM03348

biomaterial_provider

Coriell

datatype

DnaseSeq

datatype description

DNaseI HS Sequencing

labversion

baseAlignCounts.pl v 1,fseq v 1.84, iff_generic_male,p-value cutoff: 0.05

replicate

1,2

cell

Fibrobl_GM03348

cell organism

human

cell description

skin fibroblast

cell karyotype

normal

cell sex

M

treatment

lenti-MyoD

treatment description

GM03348 skin fibroblasts (Coriell) were transduced with an induceable lentivirus (pAK111-TRE-3xflag hMyoD-T2A-dsRed) containing get inducible MyoD. Cells were selected in 1ug per ml puro (Sigma catalog P8833) to obtain a pure cell population. Cells were induced for MYOD expression for 10 days with doxycycline and at concentration of 3ug per ml. Standard media was used (and replaced every 2 days) during induction: DMEM, 1% Pen Strep, and 10% FBS. (Crawford)

labversion

baseAlignCounts.pl v 1

Sequenced DNA Library

library_name

GSM1008568: Duke_DnaseSeq_Fibrobl_GM03348_lenti-MyoD

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

415755912

Reads aligned (%)

0.0

Duplicates removed (%)

12.5

Number of peaks

182 (qval < 1E-05)

hg19

Number of total reads

415755912

Reads aligned (%)

0.0

Duplicates removed (%)

12.7

Number of peaks

192 (qval < 1E-05)