Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Liver
tissue
Liver
flow_cell
30393455
Sex
male
strain
C57BL6/6JRj

Sequenced DNA Library

library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
DNA isolation was performed using standard phenol chloroform methods. Bisulfite padlock probes were designed using the ppDesigner 2.0 software (22306810) for mouse chromosome 7 (mm10). The probe and library preparation was performed based on previously published protocol (ibid).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
4567778
Reads aligned (%)
100.0
Coverage rate (×)
0.4
Number of hyper MRs
1781 (qval < 1E-05)

mm9

Number of total reads
4567778
Reads aligned (%)
99.9
Coverage rate (×)
0.4
Number of hyper MRs
1779 (qval < 1E-05)

Base call quality data from DBCLS SRA