Antigen

Antigen Class

TFs and others

Antigen

ELF1

Cell type

Cell type Class

Digestive tract

Cell type

COLO 320

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Colorectal cancer cell line

cell line

COLO320

cell type

Colorectal cancer cell line

genetic modification

none

library strategy

transcription factor ChIP-seq

chip antibody

anti-ELF1 (Santa Cruz Biotechnology, sc-631X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were purified using QIA quick column (DNase I-seq, ATAC-seq, Targeted RNA-/DNA-seq, and 4C-seq), QIA minElute column (ChIP-seq) or SPRI beads (ATAC-seq). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

44731422

Reads aligned (%)

98.3

Duplicates removed (%)

8.3

Number of peaks

10539 (qval < 1E-05)

hg19

Number of total reads

44731422

Reads aligned (%)

97.6

Duplicates removed (%)

9.2

Number of peaks

10698 (qval < 1E-05)