Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Digestive tract
Cell type
LoVo
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Colorectal cancer cell line
cell line
LoVo
cell type
Colorectal cancer cell line
genetic modification
none
library strategy
histone ChIP-seq
chip antibody
anti-H3K27ac (Diagenode, C15410196)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were purified using QIA quick column (DNase I-seq, ATAC-seq, Targeted RNA-/DNA-seq, and 4C-seq), QIA minElute column (ChIP-seq) or SPRI beads (ATAC-seq). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
90931698
Reads aligned (%)
98.5
Duplicates removed (%)
79.2
Number of peaks
3341 (qval < 1E-05)

hg19

Number of total reads
90931698
Reads aligned (%)
97.8
Duplicates removed (%)
80.7
Number of peaks
5087 (qval < 1E-05)

Base call quality data from DBCLS SRA