Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Stomach

MeSH Description

An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM.

Sample

source_name

Stomach

strain background

C57BL/6 x 129 mixed

genotype/variation

wild type

age

8 weeks

tissue

Stomach

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Gastric glands were isolated from mouse antrum as described (Barker et al., 2010) and cross-linked with 1% formaldehyde for 10 min at room temperature. Chromatin fractions were sheared by sonication. ChIP-enriched and input DNA were purified and genomic libraries were prepared as described (Sulahian et al., 2014), using four micrograms of goat anti-SOX2 (AF2018, R&D). Genomic libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21270194

Reads aligned (%)

99.9

Duplicates removed (%)

22.6

Number of peaks

134 (qval < 1E-05)

mm9

Number of total reads

21270194

Reads aligned (%)

99.9

Duplicates removed (%)

22.6

Number of peaks

136 (qval < 1E-05)