GSM2224435: Control -Dox Runx1-rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Runx1
Cell type
Cell type Class
Blood
Cell type
416B
Tissue
Blood
Lineage
cellLine
Description
myeloid progenitor cells, CD34+
Attributes by original data submitter
Sample
source_name
Control_-Dox_Runx1
cell line
myeloid progenitor 416B cell line
construct
Control-ATG
treatment
None
chip antibody
Runx1 (Abcam, ab23980)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei following crosslinking with 1% formaldehyde, and histone-DNA complexes were isolated with antibody. Sequencing libraries were prepared using the TruSeq Kit (Illumina) for high throughput sequencing on an Illumina HiSeq 2000, according to manufacturer’s instructions, with size selection for fragments of 150-400 bp.