GSM6910220: ChIPseq KO3A MECP2 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MECP2
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
breast
tissue
breast
cell line
MCF-7
cell type
epithelial type of breast cancer cell
antibody
anti-MECP2 antibody
genotype
KO3A
Sequenced DNA Library
library_name
GSM6910220
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HA-FOXA1-WT and HA-FOXA1-3A cells were maintained in RPMI-1640 medium with 10% fetal calf serum. Approximately 2 x 10e7 cells were used for ChIP-seq assay. For HA-FOXA1-WT and HA-FOXA1-3A ChIP-seq, crosslinked chromatin complexes were immunoprecipitated with anti-HA-magnetic beads. For MECP2 ChIP-seq, crosslinked chromatin complexes were immunoprecipitated with anti-MECP2 antibody. For RNA-seq, total RNA was extracted from the HA-FOXA1-WT and HA-FOXA1-3A expressing cells using Trizol reagent (Invitrogen). RNA quality was checked by Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA libraries were prepared for sequencing using standard protocols. DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptor. PCR amplification and size selection (usually 100-300bp, including adaptor sequence. Qualified library for sequencing. RNA-seq libraries were constructed using SMARTer cDNA library construction kit.