SRX188942
GSM1006865: UT-A ChipSeq MCF-7 serum starved media Pol2

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
controlid
wgEncodeEH000589
labversion
baseAlignCounts.pl v 1,fseq v 1.84, iff_MCF7,p-value cutoff: 0.05
replicate
1,2
cell sex
F
treatment
serum_starved_media
treatment description
Grown with normal serum levels (10%), then switched to media containing low level of FBS (0.05%) for 72 hours
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
controlid
wgEncodeEH000589
labversion
baseAlignCounts.pl v 1

Sequenced DNA Library

library_name
GSM1006865: UT-A_ChipSeq_MCF-7_serum_starved_media_Pol2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
76971399
Reads aligned (%)
97.1
Duplicates removed (%)
20.0
Number of peaks
89174 (qval < 1E-05)

hg19

Number of total reads
76971399
Reads aligned (%)
96.7
Duplicates removed (%)
20.2
Number of peaks
89514 (qval < 1E-05)

Base call quality data from DBCLS SRA