GSM6909687: A549-PAXIP1KO-rep2+Dex(100nM) [PAXIP1 8]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PAXIP1
Cell type
Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
A549
cell type
Lung Cancer
cell line
A549
genotype
PAXIP1-KO
treatment
100nM Dexamethasone
treatment length
2hours
chip antibody
PAXIP1(ab70434, abcam)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6909687
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation followed by sequencing (ChIP)-seq was performed as previously described (Schmidt, D. et al. Methods 48, 240–248 (2009).In brief, cells were fixed in 1% formaldehyde for 10 min and quenched with 0.125M glycine. Nuclear lysates were extracted as previously described and sonicated for 13 cycles (30 sec on/30 sec off) using the Bioruptor® Pico (B01060001, Diagenode). ). Per ChIP, 50 µL of magnetic Protein A beads (10008D, Thermo Fisher Sci-entific) beads were conjugated to 7.5µL of GR antibody, or 5µg of RAD21, PAXIP1, and H3K4me1 antibodies. Immunoprecipitated DNA was processed for library preparation usin KAPA library preparation kit, KK8234, Roche.