GSM6900275: RC43T cells, VDR D3 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
VDR
Cell type
Cell type Class
Prostate
Cell type
RC43N
NA
NA
Attributes by original data submitter
Sample
source_name
RC43T
tissue
RC43T
cell line
RC43T
cell type
WT
genotype
African American prostate cancer
chip antibody
VDR
treatment
1α,25(OH)2D3 (100 nM, 6h)
Sequenced DNA Library
library_name
GSM6900275
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, approximately 20x106 cells were crosslinked with 1% formaldehyde solution, quenched with glycine (0.125 M) and harvested in cold PBS. Sonication of crosslinked chromatin was performed using a Bioruptor® UCD-200TM Sonicator (Diagenode) with optimized cycles for each cell type. Immunoprecipitation of sonicated material was performed with antibodies against VDR (D2K6W) – Cell Signaling) or IgG (Rabbit IgG sc2729 – Santa Cruz) for 16 hours, and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed, reverse crosslinked, and treated sequentially with RNase and proteinase K prior to DNA isolation. Sequencing libraries prepared from immunopreciptiated DNA