Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Others
Cell type
Fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
Fibroblast cell line, PAX3-FOXO1 vector added
cell line
7250
cell type
fibroblast
antibody
PAX3-FOXO1 mAb (not commercially available)
enrichment target
PAX3-FOXO1
target function
Fusion Transcription Factor

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
45574260
Reads aligned (%)
79.8
Duplicates removed (%)
78.5
Number of peaks
1163 (qval < 1E-05)

hg19

Number of total reads
45574260
Reads aligned (%)
79.5
Duplicates removed (%)
78.9
Number of peaks
1083 (qval < 1E-05)

Base call quality data from DBCLS SRA